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Dna Repair
Nucleotide Sequence of DNA
Introduction:
Determination of the nucleotide sequence of DNA's is difficult since even the smallest DNA molecules contain at least 5,000 nucleotide units and thus are very much longer than t RNA and 5S RNA. The deoxy-nucleases are very less specific for certain inter nucleotide linkages than the ribo- nucleases. However there have been some significant attempts to research on the same.
DNA Sequencing can be defined as a representation of the nucleotides of DNA that constitute the DNA strand. It is a prime process which involves various methods to unravel the biological and genetic aspects. Sequencing is used to find the order of the different bases of nucleotides-A, T,G , C.DNA sequencing has vastly influenced and accelerated research activities.
Allan and Walter were responsible for developing a method for DNA sequencing which was based on chemical modification of DNA and cleavage at specific sites.
A newly discovered class of deoxy-ribo nucleases called restriction-endo nucleases have been found to be very useful for specific fragmentation of DNA. These species-specific bacterial enzymes can cleave at certain specific points of DNA molecules other than those naturally present in the cells from which are derived. Restriction endo nucleases function biologically to protect a given organism from the deleterious effects of a foreign DNA introduced into the cell. Another approach to the sequencing of DNA specimens is made possible by chemical modification of DNA-to yield apurinic or apyrimidinic acids.
The restriction enzymes are endo nucleases which recognize and cleave the foreign DNA at the same specific sequence of bases as in the host-cell DNA which has been modified by methylation : the methyl groups of the host-cell DNA prevent the attack of the restriction endonucleases. The restriction endonucleases produce a double-strand break which cannot be repaired , as a result, the foreign DNA is degraded further by other nucleases in the cell.
By means of hybridisation tests it is possible to determine whether two given specimens of DNA have blocks or segments of complementary base sequences and what their extent may be .This results in a greater tendency for a given pair of DNA strands to associate and to form duplexes.
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explain how muagens can cause genetic mutations in spite of your bodys dna repair enzymes?
Explain why you think the body's DNA repair enzymes would make it unlikely.
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